1.5 P.A.C.E. contact hour(s)

(based on 393 customer ratings)

Course provided by LabCE.

See more courses in: Molecular Pathology

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-101-18, approved through 12/31/2020
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Molecular Pathology): 1.5 hour(s)
Course number 20-642445, approved through 9/1/2022


  • Define the basic steps of polymerase chain reaction (PCR).
  • Describe the basic components of singleplex and multiplex assays.
  • Discuss the advantages of multiplex PCR assays.
  • Address the challenges of multiplex PCR.
  • Outline the steps involved in validating a multiplex PCR assay.
  • Define the use of diagnostic applications for multiplex PCR assays in the laboratory.

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(based on 393 customer ratings)

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Course Outline

  • Course Introduction
      • The Need to Understand Basic Molecular Testing
  • Brief Introduction to Polymerase Chain Reaction (PCR)
      • Brief Introduction to Polymerase Chain Reaction (PCR)
  • Define Singleplex vs. Multiplex Assay
      • Singleplex versus Multiplex Assay
  • Advantages of Multiplex PCR Assays
      • Advantages of Multiplex PCR
  • Multiplex PCR Primer Design and Additional Reagents
      • Primer Design
      • Basic Rules of Primer Design
      • Melting Temperature (Tm)
      • Annealing Temperature (Ta)
      • Optimization of dNTPs, MgCl2, Polymerase, and Salts
      • Primer Design Summary Table
  • Multiplex PCR Verification and Validation
      • Definitions
    • Sample Transport and Processing Criteria
      • Sample Transport and Processing Criteria
    • Assay Verification and Validation Parameters
      • Validation Material
      • Assay Verification and Validation
      • Define Targets
      • Analytical Sensitivity and Specificity
      • Accuracy and Precision
      • Instrumentation: Test System and Platform
      • Summary of Validation Criteria
  • Assay Analytical Perspectives
      • Assay Performance
      • Impact of Prevalence on Sensitivity and Specificity
  • Applications of Infectious Disease Multiplex PCR
      • Advantages for Using Multiplex PCR Assays for Certain Infectious Syndromes
  • References
      • References
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