Chemical Screening of Urine by Reagent Strip (by LabCE)

1.5 P.A.C.E. contact hour(s)

(based on 1,481 customer ratings)

Author: Leah Coppolino, MPH, MLS(ASCP)
Reviewer: Alexandru Casapu, MBA, MLS(ASCP)CM

Course provided by LabCE.

This course discusses the chemical tests that are an integral part of a urinalysis. Proper specimen handling and technique are discussed for key tests, including pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, and leukocyte esterase.

See more courses in: Clinical Chemistry / Urinalysis / Toxicology

Included In These Course Packages

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-018-20, approved through 2/28/2022
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 1.5 hour(s)
Course number 20-689037, approved through 9/1/2022

Objectives

  • Define the optimal storage time and temperature for urinalysis samples.
  • Review the proper storage of and procedure for the use of urinalysis reagent strips.
  • Describe the chemical reactions, quality control measures, and interpretation of results for urinalysis reagent strip analytes including pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, leukocyte esterase.
  • Explain the proper techniques used during urinalysis reagent strip testing; ensuring accuracy.
  • Identify conditions and/or substances that may interfere with urinalysis reagent strip testing.
  • Identify the additional testing protocols used to confirm urinalysis reagent strip testing results.
  • Summarize the analytical principles of measurement in urinalysis reagent strip instruments.

Customer Ratings

(based on 1,481 customer ratings)

Course Outline

  • Introduction to Urine Chemical Reagent Strips
      • Chemical Urinalysis Reagent Strips
      • Urine Specimen Collection
      • Urine Specimen Transport
      • Reagent Strip Procedure: Manual or Semi-Automated Methods
      • Handling and Storage of Urine Reagent Strips
  • Urine pH Analysis
      • pH Analysis: The Urine Specimen
      • pH Reaction
      • Urine pH: Acidic and Alkaline
  • Urine Specific Gravity Analysis
      • Measuring Specific Gravity (SG)
      • Advantages and Limitations of the Chemical Reagent Strip Method for Specific Gravity (SG)
      • Clinical Significance of Specific Gravity
  • Urine Protein Analysis
      • Protein Error of Indicators
      • Confirmatory Testing for Urine Protein
      • Clinical Significance of Urine Protein
      • Clinical Significance of Urine Protein, continued
      • Follow-up Testing for Positive Urine Protein
  • Urine Glucose Analysis
      • Glucose Reaction
      • False-Positive and False-Negative Urine Glucose Results
      • Clinical Significance of Glucose in the Urine
  • Urine Ketone Analysis
      • Ketones Overview
      • Ketone Reaction
      • False-Positive and False-Negative Ketone Test Results
      • Clinical Significance of Urine Ketones
  • Urine Blood Analysis
      • Introduction to Hematuria
      • Blood Reaction
      • False-Positive and False-Negative Results
      • Clinical Significance of Blood in Urine
  • Urine Nitrite Analysis
      • Nitrite
      • Nitrite Reaction
      • Nitrite Test Sensitivity
      • False-Positive and False-Negative Results
      • Clinical Significance of Nitrite in Urine
  • Urine Leukocytes
      • Leukocytes Reaction
      • Leukocytes Reaction, continued
      • False-Positive and Falsely-Decreased Results
      • Clinical Significance of Leukocytes in Urine
  • Urine Bilirubin Analysis
      • Overview of Bilirubin
      • Bilirubin Reaction
      • False-Positive and False-Negative Urine Bilirubin Results
      • Ruling out False-Positive Urine Bilirubin Test Results
      • Clinical Significance of Urine Bilirubin
  • Urine Urobilinogen Analysis
      • Introduction to Urobilinogen
      • Urobilinogen Reaction
      • False-Positive and False-Negative Urobilinogen Results
      • Clinical Significance of Urobilinogen in Urine
  • Automation of Chemical Reagent Strip Analysis
      • Semi-Automated Instruments
      • Automated Systems
      • Quality Control
  • References
      • References

Additional Information

Level of instruction: Basic
 
Intended audience: Medical laboratory science and medical laboratory technician students, clinical laboratory personnel, and other medical personnel who perform urine dipstick testing at the point-of-care.  
 
Author information: Leah Coppolino, MPH, MLS(ASCP) is the Director of Sales and Marketing for MediaLab, Inc. Previously, she was the Director of Education and Outreach as well as the Medical Laboratory Science Program Director at St. Christopher’s Hospital for Children in Philadelphia, Pennsylvania. Leah holds a Masters in Public Health from Thomas Jefferson University.
 
Reviewer Information: Alexandru Casapu, MBA, MLS(ASCP)CM, PBTCM has over 20 years of experience as a medical laboratory scientist, section supervisor, and laboratory manager. He is the former Director of Clinical Laboratory Technology Program at Georgia Piedmont Technical College. He is currently a Program Director at MediaLab, Inc. Alexandru holds BS degrees in Biology and Medical Technology from Clark Atlanta University and a MBA from the University of Georgia.
 

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Course provided by LabCE.
2 urine microscopic tubes in blue rack sml


dye interference


orange lid sterile urine cup with urine 2 sml


urine strip almost dipping into urine cup sml


urine strips with controls sml


timer for urinalysis sml


Correct storage


urine strip vertical in front of strip bottle and urine sml



 
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