(based on 176 customer ratings)

Course provided by LabCE.

See more courses in: Histology

Continuing Education Credits

Course number 20-636381, approved through 9/1/2022

Objectives

  • Understand basic tissue staining methods used in the clinical histology laboratory.
  • Explain factors that affect dye binding and use to troubleshoot staining issues.
  • Classify nervous tissue elements and identify which dyes will bind to these elements.
  • Demonstrate safe microwave use to perform special staining procedures.

Customer Ratings

(based on 176 customer ratings)

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Course Outline

  • Introduction
      • Tissue Staining in the Clinical Histology Laboratory
      • The Biological Stain Commission (BSC)
  • Basic Staining Mechanisms
      • What Makes a Dye?
      • Factors that Affect Dye Binding
      • Acids, Bases, pH, and Buffers
      • Basophilic and Acidophilic Staining
      • Oxidation and Reduction
  • Nervous System Overview
      • Classifying Nervous Tissue
      • Functions of Nervous Tissue
      • Neurons: A Visual Overview
      • Histology of the Nervous System: Neurons
      • Histology of the Nervous System: Neurofibrillary Tangles (NFTs)
      • Histology of the Nervous System: Nissl Substance
      • Histology of the Nervous System: Neuroglia
      • Histology of the Nervous System: Myelin
  • Special Staining Methods Used to Demonstrate Nervous Tissue
      • Classifying Nervous Tissue Staining Methods
      • Cresyl Echt Violet Staining - Chemistry
      • Cresyl Echt Violet Staining - Diagnostic Applications
      • Cresyl Echt Violet Staining - Staining Protocol
      • Bielschowsky Silver Staining - Chemistry
      • Bielschowsky Silver Staining - Diagnostic Applications
      • Bielschowsky Silver Staining - Staining Protocol
      • Luxol Fast Blue (LFB) Staining - Chemistry
      • Luxol Fast Blue (LFB) Staining - Diagnostic Applications
      • Luxol Fast Blue (LFB) Staining - Staining Protocol
      • Holzer's Crystal Violet Staining - Chemistry
      • Holzer's Crystal Violet Staining - Diagnostics
      • Holzer's Crystal Violet Staining - Staining Protocol
      • Luxol Fast Blue (LFB) - Cresyl Echt Violet Staining - Chemistry
      • Luxol Fast Blue (LFB) - Cresyl Violet Staining - Diagnostic Applications
      • Luxol Fast Blue (LFB) - Cresyl Violet Staining - Staining Protocol
  • Using the Microwave for Special Staining Procedures
      • Microwaves in the Histopathology Laboratory
      • Tips for Using the Microwave for Special Staining Procedures
      • Safety Precautions for Microwave Usage
  • References
      • References
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Course provided by LabCE.

 
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