(based on 222 customer ratings)

Course provided by LabCE.

See more courses in: Histology

Continuing Education Credits

Course number 20-684414, approved through 9/1/2022


  • Understand basic tissue staining methods used in the clinical histology laboratory.
  • Explain factors that affect dye binding and utilize to troubleshoot staining issues.
  • Classify connective tissue elements and identify which dyes will bind to these elements.
  • Demonstrate safe microwave use when necessary to perform special staining procedures.

Customer Ratings

(based on 222 customer ratings)

Course Outline

  • Introduction
      • Tissue Staining in the Clinical Histology Laboratory
      • The Biological Stain Commission (BSC)
  • Basic Staining Mechanisms
      • What Are Dyes?
      • Factors That Affect Dye Binding
      • Acids, Bases, pH, and Buffers
      • Basophilic and Acidophilic Staining
      • Oxidation and Reduction
  • Connective Tissue Classification and Functions
      • What is Connective Tissue?
      • Classifying Connective Tissue
      • Functions of Connective Tissue
  • Special Staining Methods Used to Demonstrate Connective Tissue
      • Gordon and Sweet's Silver Stain - Chemistry
      • Gordon and Sweet's Silver Stain - Diagnostic Applications
      • Gordon and Sweet's Silver Stain - Staining Protocol
      • Jones' Methenamine Silver Stain (JMS) - Chemistry
      • Jones' Methenamine Silver Stain (JMS) - Diagnostic Applications
      • Jones' Methenamine Silver Stain (JMS) - Staining protocol
      • Verhoeff-Van Gieson (VVG) Stain - Chemistry
      • Verhoeff-Van Gieson (VVG) Stain - Diagnostic Applications
      • Verhoeff-Van Gieson (VVG) Stain - Staining Protocol
      • Masson's Trichrome Stain - Chemistry
      • Masson's Trichrome Stain - Diagnostic Applications
      • Masson's Trichrome Stain - Staining Protocol
      • Phosphotungstic Acid-Hematoxylin (PTAH) Stain - Chemistry
      • Phosphotungstic Acid-Hematoxylin (PTAH) Stain - Diagnostic Applications
      • Phosphotungstic Acid-Hematoxylin (PTAH) Stain - Staining Protocol
  • Using the Microwave for Special Staining Procedures
      • Microwaves in the Histopathology Laboratory
      • Tips for using the Microwave for Special Staining Procedures
      • Safety Precautions for Microwave Usage
  • References
      • References
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Course provided by LabCE.

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