This course does not carry any current continuing education credits.

(based on 169 customer ratings)

Course provided by LabCE.

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  • Differentiate between natural and synthetic dyes.
  • Analyze progressive staining versus regressive staining.
  • Distinguish between the oxidants and mordants in common hematoxylin and eosin (H&E) stains.
  • Identify cell constituents demonstrated with the H&E stain.
  • Describe the purpose of and reagents used for deparaffinization, hydration, and dehydration.
  • Identify potential problems encountered with routine staining and solutions to resolve them.

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(based on 169 customer ratings)

Course Outline

  • Introduction
      • Clinical Significance and Correlation of Histology
      • How is Histology Useful to the Pathologist?
      • Application of the Hematoxylin and Eosin (H&E) Stain
      • Why is the H&E Stain the Most Common?
  • Categories of Biological Stains
      • Three Broad Categories of Biological Stains
  • Classification of Biological Stains
      • Classification of Biological Stains
      • Origin of Dyes: Natural
      • Origin of Dyes: Synthetic
      • Examples of Synthetic Dyes
      • Chemistry of Dyes
      • Mechanisms of Action
      • Progressive or Regressive Hematoxylin Staining
  • Routine Staining in the Histology Laboratory
      • Hematoxylin Oxidation
      • Mordants
      • Commonly used Hematoxylins: Their Oxidizers and Mordants
      • Differentiating
      • The Bluing Step
      • Eosin as a Counterstain
      • Types of Eosin
      • Eosin Differentiation
  • Applications of the H&E Stain
      • Healthy Versus Diseased Tissue with H&E Stain
      • Uterus
      • Liver
      • Breast
      • Skin
      • Appendix
      • Fallopian Tube
  • Additional Steps Employed in Routine H&E Staining
      • Paraffin Sections
      • Paraffin Sections, continued:
      • Staining Time Comparisons
      • General H&E Staining Procedures
  • Some Common Problems Encountered in H&E Staining
      • Nuclear Staining Errors
      • Poor Eosin Differentiation
      • Poor Contrast Between the Hematoxylin and the Eosin
      • Reddish- Brown Nuclei
      • Dark Precipitate On The Slide
      • Uneven Staining
      • White Patches on Slides After Deparaffinization Step
      • Clear Patches on Tissue After Hydrating
      • Contaminated Clearing Agent
  • References
      • References
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Course provided by LabCE.

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